Journal: eLife

Article Title: Controlling motor neurons of every muscle for fly proboscis reaching

doi: 10.7554/eLife.54978

Figure Lengend Snippet:

Article Snippet: Antibody , rat mAb anti-FLAG , Novus Biologicals , Cat# NBP1-06712; RRID: AB_1625981 , (1:200) , .

Techniques: Muscles, Construct, Software

Journal: Cell host & microbe

Article Title: Single Particle Imaging of Polarized Hepatoma Organoids upon Hepatitis C Virus Infection Reveals an Ordered and Sequential Entry Process

doi: 10.1016/j.chom.2018.02.005

Figure Lengend Snippet: (A–D) Huh-7.5 organoids were incubated with 15 mM Erlotinib (Erl) or DMSO for 1 hr, infected with DiD-HCV for 1 hr at 4C, shifted to 37°C for the indicated times, fixed, and probed for claudin-1 (A) or core (C). (A) Tight junction region is shown in the inset. Left: claudin (green), Right: DiD-HCV (red). (B) Quantitation of (A). (C) Arrows indicate DiD-HCV particles enlarged in insets; dashed arrows and arrowhead represent colocalization with core. Left: core (green); right: DiD-HCV (red). Lack of DiD-HCV colocalization with core antibody, seen in DMSO treated cluster at 360 min post shift, indicates an uncoating event. (D) Quantitation of (C). (E and F) Huh-7.5, shEGFR, or shEGFR+EGFR organoids were infected with DiD-HCV as above, fixed at indicated times, and stained for ZO-1. (E) Tight junction region is shown in the inset. Left: ZO-1 (green); right: DiD-HCV (red). (F) Quantitation of (E). n = total DiD signal, mean ± SD. **p < 0.01, ***p < 0.001. See also Figure S5.

Article Snippet: Inhibitor concentrations were as follows: 10 mM Cytochalasin D (Sigma), 15 mM Erlotinib (Santa Cruz) and 20 mM Ammonium Chloride (Fisher).

Techniques: Incubation, Infection, Quantitation Assay, Staining

Journal: Cell host & microbe

Article Title: Single Particle Imaging of Polarized Hepatoma Organoids upon Hepatitis C Virus Infection Reveals an Ordered and Sequential Entry Process

doi: 10.1016/j.chom.2018.02.005

Figure Lengend Snippet: Huh-7.5 organoids were incubated with either erlotinib or vehicle control (VC) for 1 hr, if indicated, infected with DiD-HCV for 1 hr at 4C, shifted to 37°C for stated times, fixed, and probed for clathrin light chain (clathrin LC) (A and C) or Rab5a (E), both in green. Arrows mark areas enlarged in insets; dashed arrows and arrowheads denote colocalization with labeled host factor. (C) Organoids seeded with Huh-7.5 or Huh-7.5-shEGFR cells were infected with DiD-HCV as above, fixed, and stained for clathrin LC. (B, D, and F) Quantitation of (A), (C), and (E), respectively. n = total DiD signal; error bars, SD. *p < 0.05, **p < 0.01, ***p < 0.001. See also Figure S6.

Article Snippet: Inhibitor concentrations were as follows: 10 mM Cytochalasin D (Sigma), 15 mM Erlotinib (Santa Cruz) and 20 mM Ammonium Chloride (Fisher).

Techniques: Incubation, Control, Infection, Labeling, Staining, Quantitation Assay

Journal: Cell host & microbe

Article Title: Single Particle Imaging of Polarized Hepatoma Organoids upon Hepatitis C Virus Infection Reveals an Ordered and Sequential Entry Process

doi: 10.1016/j.chom.2018.02.005

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Inhibitor concentrations were as follows: 10 mM Cytochalasin D (Sigma), 15 mM Erlotinib (Santa Cruz) and 20 mM Ammonium Chloride (Fisher).

Techniques: Control, Virus, Recombinant, Cell Recovery, Cell Viability Assay, Plasmid Preparation, shRNA, Software